Description of the CFS Course

 

The course lectures are prepared according to specific topics in fluorescence spectroscopy, and not according to fields of research. So far there are lectures on:

            Part 1-Introduction to Fluorescence Spectroscopy

            Part 2-Instrumentation, Spectra and Quantum Yields

            Part 3- Introduction to Time-resolved Fluorescence: Time-Domain Measurements

            Part 4- Introduction to Frequency-domain Time-resolved Fluorescence

            Part 5- Fluorescence Probes

            Part 6-Solvent and Environmental Effects on Fluorescence

            Part 7- Advanced Topics in Fluorescence Resonance Energy Transfer (FRET)

            Part 8-Quenching of Fluorescence

            Part 9-Introduction to Fluorescence Anisotropy

            Part 10-Fluorescence Polarization Assays

            Part 11-Fluorescence Sensing

            Part 12- Protein Fluorescence 

            Part 13- Fluorescence Lifetime Imaging Microscopy

The individual lectures are about 30 minutes long. In each lecture we describe the basic principles of the topic, and then select biochemical and biomedical examples of the method. The lectures also describe the most common errors in the measurement.The lectures are suitable for a graduate-level course on all aspects of fluorescence.

 

In our department, we plan to view each lecture with students in the classroom, with the presence of a faculty monitor to answer questions. We are considering a list of questions for each lecture.

 

Joseph R. Lakowicz, Ph.D.

Baltimore, January 2025